Pathogenetic role of tumor necrosis factor (TNF-α) for the development of peritoneal tuberculosis in an experiment

Author:

Plotkin D. V.1ORCID,Vinogradova T. I.2ORCID,Reshetnikov M. N.3ORCID,Zyuzya Yu. R.3ORCID,Okovityi S. V.4ORCID,Sinitsyn M. V.3ORCID,Gaitukaev V. R.5ORCID,Rodoman G. V.6ORCID,Bogorodskaya E. M.3ORCID,Yablonskiy P. K.2ORCID

Affiliation:

1. Moscow Research and Clinical Center for TB Control, Moscow Healthcare Department; Pirogov Russian National Research Medical University of the Ministry of Healthcare of the Russian Federation

2. Saint Petersburg State Research Institute of Phthisiopulmonology of the Ministry of Healthcare of the Russian Federation

3. Moscow Research and Clinical Center for TB Control, Moscow Healthcare Department

4. Saint Petersburg State Chemical Pharmaceutical University of the Ministry of Healthcare of the Russian Federation

5. OLLA-MED Company

6. Pirogov Russian National Research Medical University of the Ministry of Healthcare of the Russian Federation

Abstract

Currently tuberculosis is considered as a group of diseases united by one etiological factor. The pathogenesis of certain localizations of tuberculous inflammation, in particular peritoneum tuberculosis, hasn’t been sufficiently studied. The role of cytokine mechanisms in the development of the disease and the elaboration of non-sterile immunity requires further experimental studies, in particular the creation of a reproducible model on laboratory animals.The aim: to study the effect of TNF-α on the development of tuberculosis of the serous coat of the abdominal cavity, as well as to evaluate the possibility of modeling tuberculous peritonitis in laboratory animals using infliximab.Materials and methods. The studies were conducted on 18 male rabbits, which were simulated peritoneal tuberculosis by intra-abdominal administration of a suspension of Mycobacterium tuberculosis. 10 rabbits of the experimental group were intravenously injected with an infliximab solution and an iron (III) hydroxide sucrose complex intraperitoneally a day before infection.Results. In the control group of animals, tuberculosis either didn’t develop, or in a third of cases it affected only the pulmonary parenchyma, while proliferative processes prevailed. On the contrary, in animals with inactivated TNF-α, in 100 % of observations, tuberculous peritonitis was detected with associated lung damage and the predominance of alterative caseous processes.Conclusion. The created model of tuberculous peritonitis shows the leading role of TNF-α in the activation of macrophages, as well as in attracting cells to the site of infection. This is the primary signal necessary for the formation and stability of granulomas since the neutralization of this cytokine leads to a loss of control over the infection and the destruction of the granuloma with the development of destructive tuberculosis in the serous coat of the abdominal cavity. 

Publisher

FSPSI SCFHHRP

Subject

General Immunology and Microbiology,General Biochemistry, Genetics and Molecular Biology

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