Affiliation:
1. Akademi Farmasi Yarsi Pontianak
Abstract
Exposure to sunlight for a long time and repeatedly can also cause chemical and physical damage to human hair. Clinically, microscopic analysis using a scanning electron microscope (SEM) can assess hair damage by identifying the characteristic morphology of hair damage. This study aims to analyze the morphology of damaged hair chemically induced using 3% H2O2 and photoinduced UVB radiation before and after applying sappan wood (Caesalpinia sappan) hair tonic. The active ingredients used were ethanol extract, ethanol fraction, and chloroform-methanol fraction of C. sappan, which contains an antioxidant compound. Caesalpinia sappan simplicia was macerated using 96% ethanol and then partitioned using n-hexane. The ethanol fraction was then applied using vacuum column chromatography using chloroform : methanol (5 : 1) as eluent. The extracts and fractions were then formulated into hair tonic preparations. For SEM analysis, hair samples were coated with a sputter gold coater machine and divided into five treatments: undamaged hair, damaged hair with 3% H2O2 and UVB rays as a positive control, and treatment I, II, and III, in which the hair was damaged with 3% H2O2 and UVB rays respectively, then FI, FII, and FIII were applied, respectively (2000x magnification). Damaged hair with UVB induction shows moderate damage, and 3% H2O2 shows moderate to severe damage. The results in the treatment group show that the three hair tonics coated the hair cuticle, indicating an interaction with the hair fiber, and modified the cuticle by coating the cuticle.
Publisher
Universitas Muhammadiyah Palangkaraya
Reference27 articles.
1. 1. Lasisi T, Smallcombe JW, Kenney WL, Shriver MD, Zydney B, Jablonski NG, et al. Human scalp hair as a thermoregulatory adaptation. Proc Natl Acad Sci U S A. 2023;120(24):e2301760120. doi:10.1073/pnas.2301760120
2. 2. Wall D, Meah N, Fagan N, York K, Sinclair R. Advances in hair growth. Fac Rev. 2022;11:1. doi:10.12703/r/11-1
3. 3. Nayak BS, Ann CY, Azhar AB, Ling ECS, Yen WH, Aithal PA. A Study on Scalp Hair Health and Hair Care Practices among Malaysian Medical Students. Int J Trichology. 2017;9(2):58-62. doi:10.4103/ijt.ijt_76_16
4. 4. Fernandes C, Medronho B, Alves L, Rasteiro MG. On Hair Care Physicochemistry: From Structure and Degradation to Novel Biobased Conditioning Agents. Polymers. 2023;15(3):608. doi:10.3390/polym15030608
5. 5. Ross AB, Maes E, Lee EJ, Homewood I, Marsh JM, Davis SL, et al. UV and visible light exposure to hair leads to widespread changes in the hair lipidome. Int J Cosmet Sci. 2022;44(6):672-84. doi:10.1111/ics.12810