Isolation of viable monkeypox virus from anal and urethral swabs, Italy, May to July 2022-Reference-Cited by-同舟云学术

Isolation of viable monkeypox virus from anal and urethral swabs, Italy, May to July 2022

Published:2022-09-08 Issue:36 Volume:27 Page:
ISSN:1560-7917
Container-title:Eurosurveillance
language:en
Short-container-title:

Author:

Moschese Davide¹^ORCID,Pozza Giacomo²,Mileto Davide³,Giacomelli Andrea²,Cutrera Miriam³,Cossu Maria Vittoria¹,Matone Maddalena¹,Beltrami Martina²,Salari Federica³,Antinori Spinello⁴,Lombardi Alessandra³,Rizzardini Giuliano¹

Affiliation:

1. I Division of Infectious Diseases, Luigi Sacco Hospital, ASST Fatebenefratelli Sacco, Milan, Italy

2. III Division of Infectious Diseases, Luigi Sacco Hospital, ASST Fatebenefratelli Sacco, Milan, Italy

3. Laboratory of Clinical Microbiology, Virology and Bioemergencies, Luigi Sacco Hospital, ASST Fatebenefratelli Sacco, Milan, Italy.

4. Department of Biomedical and Clinical Sciences, Università degli Studi di Milano, Italy; III Division of Infectious Diseases, ASST Fatebenefratelli Sacco, Luigi Sacco Hospital, Milan, Italy.

Abstract

Anal and urethral samples from confirmed cases of monkeypox were screened for monkeypox virus (MPXV) by real-time PCR. Isolation of the virus was subsequently attempted in cell culture. Actively-replicating virus was demonstrated in 13 of 18 and 11 of 15 PCR-positive anal and urethral swabs, respectively, collected within 7 days from symptoms onset. Two asymptomatic secondary cases had detectable MPXV genetic material in urethral secretion and for one, MPXV was successfully isolated, supporting a potential MPXV sexual transmission hypothesis.

Publisher

European Centre for Disease Control and Prevention (ECDC)

Subject

Virology,Public Health, Environmental and Occupational Health,Epidemiology

Reference27 articles.

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