Multicentre comparison of quantitative PCR-based assays to detect SARS-CoV-2, Germany, March 2020-Reference-Cited by-同舟云学术

Multicentre comparison of quantitative PCR-based assays to detect SARS-CoV-2, Germany, March 2020

Published:2020-06-18 Issue:24 Volume:25 Page:
ISSN:1560-7917
Container-title:Eurosurveillance
language:en
Short-container-title:

Author:

Muenchhoff Maximilian¹²,Mairhofer Helga¹²,Nitschko Hans¹²,Grzimek-Koschewa Natascha¹²,Hoffmann Dieter³¹,Berger Annemarie⁴¹,Rabenau Holger⁴¹,Widera Marek⁴¹,Ackermann Nikolaus⁵,Konrad Regina⁵,Zange Sabine⁶¹,Graf Alexander⁷,Krebs Stefan⁷,Blum Helmut⁷,Sing Andreas⁵,Liebl Bernhard⁵,Wölfel Roman⁶¹,Ciesek Sandra⁴¹,Drosten Christian⁸¹,Protzer Ulrike³¹,Boehm Stephan¹²,Keppler Oliver T¹²

Affiliation:

1. German Center for Infection Research, Partner Site Munich and Associated Partner Site Charité, Berlin and Associated Partner Site Frankfurt, Germany

2. Max von Pettenkofer Institute and Gene Center, Virology, National Reference Center for Retroviruses, Ludwig Maximilian University, Munich, Germany

3. Institute of Virology, School of Medicine, Technical University Munich/Helmholtz Zentrum München, Munich, Germany

4. Institute of Medical Virology, University Hospital, Goethe University Frankfurt am Main, Frankfurt, Germany

5. Bavarian Health and Food Safety Authority, Oberschleißheim, Germany

6. Bundeswehr Institute of Microbiology, Munich, Germany

7. Laboratory for Functional Genome Analysis (LAFUGA), Gene Center, Ludwig Maximilian University, Munich, Germany

8. Institute of Virology, Charité University Medicine, Berlin, Germany

Abstract

Containment strategies and clinical management of coronavirus disease (COVID-19) patients during the current pandemic depend on reliable diagnostic PCR assays for the severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2). Here, we compare 11 different RT-PCR test systems used in seven diagnostic laboratories in Germany in March 2020. While most assays performed well, we identified detection problems in a commonly used assay that may have resulted in false-negative test results during the first weeks of the pandemic.

Publisher

European Centre for Disease Control and Prevention (ECDC)

Subject

Virology,Public Health, Environmental and Occupational Health,Epidemiology

Reference9 articles.

1. A new coronavirus associated with human respiratory disease in China.;Wu;Nature,2020

2. Centers for Disease Control and Prevention (CDC). Information for laboratories about coronavirus (COVID-19). Atlanta: CDC. [Accessed: 16 April 2020]. Available from: https://www.cdc.gov/coronavirus/2019-ncov/lab/index.html

3. Detection of 2019 novel coronavirus (2019-nCoV) by real-time RT-PCR.;Corman;Euro Surveill,2020

4. World Health Organization (WHO). Coronavirus disease (COVID-19) technical guidance: Laboratory testing for 2019-nCoV in humans. Geneva: WHO. [Accessed: 16 April 2020]. Available from: https://www.who.int/emergencies/diseases/novel-coronavirus-2019/technical-guidance/laboratory-guidance

5. Comparison of seven commercial RT-PCR diagnostic kits for COVID-19.;van Kasteren;J Clin Virol,2020

Cited by 60 articles. 订阅此论文施引文献订阅此论文施引文献，注册后可以免费订阅5篇论文的施引文献，订阅后可以查看论文全部施引文献

1. Detection of SARS-CoV-2 in aerosols in long term care facilities and other indoor spaces with known COVID-19 outbreaks;Environmental Research;2024-02

2. Interlaboratory comparison using inactivated SARS-CoV-2 variants as a feasible tool for quality control in COVID-19 wastewater monitoring;Science of The Total Environment;2023-12

3. Smallest dual-color qPCR device;Sensors and Actuators B: Chemical;2023-11

4. Temporal changes in fecal microbiota of patients infected with COVID-19: a longitudinal cohort;BMC Infectious Diseases;2023-08-18

5. Rapid Detection of SARS-CoV-2 RNA Using Reverse Transcription Recombinase Polymerase Amplification (RT-RPA) with Lateral Flow for N-Protein Gene and Variant-Specific Deletion–Insertion Mutation in S-Protein Gene;Viruses;2023-05-26