Strain-dependent variations in replication of European clade 2.3.4.4b influenza A(H5N1) viruses in bovine cells and thermal inactivation in semi-skimmed or whole milk

Author:

Palme Diana I1ORCID,Lang Juliane1,Helke Dajana1,Kuryshko Maryna1ORCID,Abdelwhab Elsayed M1ORCID

Affiliation:

1. Institute of Molecular Virology and Cell Biology, Friedrich-Loeffler-Institut, Federal Research Institute for Animal Health, Greifswald-Insel Riems, Germany

Abstract

We investigated the thermostability of four European avian influenza A(H5N1) viruses in whole and semi-skimmed milk and their replication in bovine kidney and lung cells amid the current influenza A(H5N1) dairy cattle outbreak in the United States. Results showed strain-dependent differences in thermal inactivation, particularly in whole milk, and variable replication efficacy in lung cells. These findings support assessing the inactivation of European H5N1 viruses in milk and their replication in bovine cells, aiding biosafety protocols and public health measures.

Publisher

European Centre for Disease Control and Prevention (ECDC)

Reference13 articles.

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3. Highly pathogenic avian influenza A(H5N1) clade 2.3.4.4b virus infection in domestic dairy cattle and cats, United States, 2024.;Burrough;Emerg Infect Dis,2024

4. Cow’s milk containing avian influenza A(H5N1) virus - heat inactivation and infectivity in mice.;Guan;N Engl J Med,2024

5. CDC: H5N1 bird flu confirmed in person exposed to cattle.;Harris;JAMA,2024

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