Evaluation of 11 commercially available PCR kits for the detection of monkeypox virus DNA, Berlin, July to September 2022-Reference-Cited by-同舟云学术

Evaluation of 11 commercially available PCR kits for the detection of monkeypox virus DNA, Berlin, July to September 2022

Published:2022-11-10 Issue:45 Volume:27 Page:
ISSN:1560-7917
Container-title:Eurosurveillance
language:en
Short-container-title:

Author:

Michel Janine¹^ORCID,Targosz Angelina¹,Rinner Thomas¹,Bourquain Daniel¹,Brinkmann Annika¹,Sacks Jilian Amber²,Schaade Lars¹,Nitsche Andreas¹

Affiliation:

1. Robert Koch Institute (RKI), Center for Biological Threats and Special Pathogens, German Reference Laboratory for Poxviruses, Berlin, Germany

2. World Health Organization (WHO), Department of Epidemic and Pandemic Preparedness and Prevention, Geneva, Switzerland

Abstract

Before the international spread of monkeypox in May 2022, PCR kits for the detection of orthopoxviruses, and specifically monkeypox virus, were rarely available. Here we describe the evaluation of 11 recently developed commercially available PCR kits for the detection of monkeypox virus DNA. All tested kits are currently intended for research use only and clinical performance still needs to be assessed in more detail, but all were suitable for diagnostics of monkeypox virus, with variations in specificity rather than sensitivity.

Publisher

European Centre for Disease Control and Prevention (ECDC)

Subject

Virology,Public Health, Environmental and Occupational Health,Epidemiology

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