Specific serology for emerging human coronaviruses by protein microarray-Reference-Cited by-同舟云学术

Specific serology for emerging human coronaviruses by protein microarray

Published:2013-04-04 Issue:14 Volume:18 Page:
ISSN:1560-7917
Container-title:Eurosurveillance
language:en
Short-container-title:

Author:

Reusken C¹²,Mou H³²,Godeke G J¹²,van der Hoek L⁴,Meyer B⁵,Müller M A⁵,Haagmans B⁶,de Sousa R¹,Schuurman N³,Dittmer U⁷,Rottier P³,Osterhaus A⁶,Drosten C⁵,Bosch B J³,Koopmans M¹⁶

Affiliation:

1. Centre for Infectious Disease Control, Division Virology, National Institute for Public Health and the Environment, Bilthoven, the Netherlands

2. These authors contributed equally to this work

3. Department of Infectious Diseases and Immunology, Utrecht University, Faculty of Veterinary Medicine, Utrecht, the Netherlands

4. Laboratory of Experimental Virology, Department of Medical Microbiology, Center for Infection and Immunity Amsterdam, Academic Medical Center, University of Amsterdam, Amsterdam, the Netherlands

5. Institute of Virology, University of Bonn Medical Centre, Bonn, Germany

6. Department of Viroscience, Erasmus Medical Centre, Rotterdam, the Netherlands

7. Institute for Virology, University Hospital Essen, University of Duisburg-Essen, Essen, Germany

Abstract

We present a serological assay for the specific detection of IgM and IgG antibodies against the emerging human coronavirus hCoV-EMC and the SARS-CoV based on protein microarray technology. The assay uses the S1 receptor-binding subunit of the spike protein of hCoV-EMC and SARS-CoV as antigens. The assay has been validated extensively using putative cross-reacting sera of patient cohorts exposed to the four common hCoVs and sera from convalescent patients infected with hCoV-EMC or SARS-CoV.

Publisher

European Centre for Disease Control and Prevention (ECDC)

Subject

Virology,Public Health, Environmental and Occupational Health,Epidemiology

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