Calculation of the Median Lethal Dose and Low Lethal Dose Using the CombiStats Biometric Software-Reference-Cited by-同舟云学术

Calculation of the Median Lethal Dose and Low Lethal Dose Using the CombiStats Biometric Software

Published:2021-07-03 Issue:2 Volume:11 Page:135-142
ISSN:2619-1172
Container-title:The Bulletin of the Scientific Centre for Expert Evaluation of Medicinal Products
language:
Short-container-title:Vedomosti Nauchnogo tsentra ekspertizy sredstv meditsinskogo primeneniya

Author:

Shadrin P. V.¹^ORCID,Batuashvili T. A.¹^ORCID,Simutenko L. V.¹^ORCID,Neugodova N. P.¹^ORCID

Affiliation:

1. Scientific Centre for Expert Evaluation of Medicinal Products

Abstract

The median lethal dose (LD50) and low lethal dose (LD10) are calculated in acute toxicity studies, as well as during specific activity assessment of some medicines. The aim of the study was to develop a procedure for using CombiStats to calculate LD50 and LD10. The authors proposed a step-by-step algorithm for processing bioassay results using the CombiStats biometric software (median effective dose determination model, probit analysis) with conversion of doses to simple fractions (fractions of the maximum dose) to calculate LD50 and LD10. They compared LD50 and LD10 calculation results obtained using CombiStats with those obtained using electronic spreadsheets according to the Bliss–Miller–Tainter–Prozorovsky method described in the State Pharmacopoeia of the Russian Federation (General Monograph 1.1.0014.15). It has been demonstrated that the use of CombiStats sometimes has advantages over the use of the pharmacoepoeial method.

Publisher

SCEEMP

Reference6 articles.

1. Bliss CI, McKellen C. Biological assay. Ann Rev Physiol. 1943;5:479–539. https://doi.org/10.1146/annurev.ph.05.030143.002403

2. Burn JH, Finney DJ, Goodwin LG. Biological standardization. 2nd edition. New York: Oxford University Press; 1950.

3. Babich PN, Chubenko AV, Lapach SN. Application of the probit-analysis in toxicology and pharmacology using Microsoft Excel program for the estimation of pharmacological activity in an alternative form of reactions registration. Sovremennye problemy toksikologii = Modern Problems of Toxicology. 2003;(4):80–8 (In Russ.)

4. Belen’kiy ML. Elements of a quantitative evaluation of the pharmacological effect. Leningrad: Medgiz; 1963 (In Russ.)

5. Prozorovskiy VB. Statistical processing of data of pharmacological investigations. Psikhofarmakologiya i biologicheskaya narkologiya = Psychopharmacology and Biological Narcology. 2007;7(3–4):2090–120 (In Russ.)