Affiliation:
1. Department of Cell and Molecular Biology, Faculty of Biotechnology and Biomolecular Sciences, Universiti Putra Malaysia, 43400 Serdang, Malaysia
2. Enzyme and Microbial Technology Research Center, Faculty of Biotechnology and Biomolecular Sciences, Universiti Putra Malaysia, 43400 Serdang, Malaysia
Abstract
Chitinases catalyze the degradation of chitin, a ubiquitous polymer generated from the cell walls of fungi, shells of crustaceans, and cuticles of insects. They are gaining increasing attention in medicine, agriculture, food and drug industries, and environmental management. Their roles in the degradation of chitin for the production of industrially useful products and in the control of fungal pathogens and insect pests render them attractive for such purposes. However, chitinases have diverse sources, characteristics, and mechanisms of action that seem to restrain optimization procedures and render standardization techniques for enhanced practical applications complex. Hence, results of laboratory trials are not usually consistent with real-life applications. With the growing field of protein engineering, these complexities can be overcome by modifying or redesigning chitinases to enhance specific features required for specific applications. In this review, the variations in features and mechanisms of chitinases that limit their exploitation in biotechnological applications are compiled. Recent attempts to engineer chitinases for improved efficiency are also highlighted.
Subject
Cell Biology,Molecular Biology,Biochemistry,Biophysics
Reference127 articles.
1. Fungal chitinases: function, regulation, and potential roles in plant/pathogen interactions;Langner;Curr. Genet.,2016
2. Chitin metabolism in insects: structure, function and regulation of chitin synthases and chitinases;Merzendorfer;J. Exp. Biol.,2003
3. Industrial applications of crustacean by-products (chitin, chitosan, and chitooligosaccharides): a review;Hamed;Trends Food Sci. Technol.,2016
4. Chitinolytic enzymes: An exploration;Patil;Enzyme Microb. Technol.,2000
5. Cloning, expression, purification and application of a novel chitinase from a thermophilic marine bacterium Paenibacillus barengoltzii;Yang;Food Chem.,2016
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