Affiliation:
1. Agricultural Research Council Unit of Nitrogen Fixation, University of Sussex, Brighton BN1 9QJ, U.K.
Abstract
Acetylene-reducing activity of purified nitrogenase from Klebsiella pneumoniae was studied over a range of ATP and Mg2+ concentrations at 15°C, pH7.8. Inhibition at Mg2+ concentrations of 2.5–30mm was due to the formation of the inactive complex, Mg2ATP. At higher Mg2+ concentrations an additional inhibitory effect was observed. The results were consistent with a MgATP complex being the active substrate with an apparent Km(MgATP)=0.4mm.
Subject
Cell Biology,Molecular Biology,Biochemistry
Cited by
34 articles.
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