Interaction of integrin β1 with cytokeratin 1 in neuroblastoma NMB7 cells

Abstract

Cytokeratin 1, an intermediate filament keratin, was isolated as a partner of the tyrosine kinase Src from neuroblastoma NMB7 cells. The cytokeratin 1–Src complex was found to be associated with the molecular scaffolder RACK1 (receptor for activated protein kinase C). Interestingly, the cytokeratin 1–Src–RACK1 complex was found to actively bind with membrane receptors such as integrin β1. We are interested in using this complex to find downstream kinases and phosphatases that bind upon cytokine stimulation, especially during neurogenesis.