Affiliation:
1. Children's Hospital Oakland Research Institute, 747 52nd Street, Oakland, CA 94609, U.S.A.
Abstract
Thioesterase I, a constituent domain of the multifunctional fatty acid synthase, and thioesterase II, an independent monofunctional protein, catalyse the chain-terminating reaction in fatty acid synthesis de novo at long and medium chain lengths respectively. The enzymes have been cloned and expressed in Escherichia coli under the control of the temperature-sensitive lambda repressor. The recombinant proteins are full-length catalytically competent thioesterases with specificities indistinguishable from those of the natural enzymes.
Subject
Cell Biology,Molecular Biology,Biochemistry
Cited by
42 articles.
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