A preliminary crystallographic investigation of avidin

Author:

Green N. M.1,Joynson M. A.1

Affiliation:

1. National Institute for Medical Research, Mill Hill, London N.W.7, U.K., and Laboratory of Molecular Biophysics, Department of Zoology, University of Oxford, Oxford OX1, 3QU, U.K.

Abstract

Avidin was crystallized in various forms from 3m-ammonium sulphate or sodium phosphate at pH5.2. X-ray-crystallographic analysis of crystals from phosphate showed them to be orthorhombic, space group C222, containing two molecules per unit cell and with a single subunit as the asymmetric unit. The formation of the complex with biotin did not affect the morphology of the crystals.

Publisher

Portland Press Ltd.

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