The unfolding and refolding of pig heart fumarase-Reference-Cited by-同舟云学术

The unfolding and refolding of pig heart fumarase

Published:1991-05-01 Issue:3 Volume:275 Page:745-749
ISSN:0264-6021
Container-title:Biochemical Journal
language:en
Short-container-title:

Author:

Kelly S M¹,Price N C¹

Affiliation:

1. Department of Biological and Molecular Sciences, University of Stirling, Stirling FK9 4LA, Scotland, U.K.

Abstract

The unfolding of pig heart fumarase in solutions of guanidinium chloride (GdnHCl) has been examined. Loss of activity occurs at lower concentrations of GdnHCl than the structural changes detected by fluorescence or c.d. After denaturation, regain of activity can be observed provided that a reducing agent (dithiothreitol) is present and that the concentration of GdnHCl is lowered by dialysis rather than by dilution. The regain of secondary structure occurs with high efficiency even when little or no activity is recovered.

Publisher

Portland Press Ltd.

Subject

Cell Biology,Molecular Biology,Biochemistry

Link

https://portlandpress.com/biochemj/article-pdf/275/3/745/604180/bj2750745.pdf

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