The E1 α and β Subunits of the Pyruvate Dehydrogenase Complex are M2‘d’ and M2‘e’ Autoantigens in Primary Biliary Cirrhosis-Reference-Cited by-同舟云学术

The E1 α and β Subunits of the Pyruvate Dehydrogenase Complex are M2‘d’ and M2‘e’ Autoantigens in Primary Biliary Cirrhosis

Published:1989-10-01 Issue:4 Volume:77 Page:365-368
ISSN:0143-5221
Container-title:Clinical Science
language:en
Short-container-title:

Author:

Fussey Shelley P. M.¹,Bassendine Margaret F.²,Fittes Dorothy¹,Turner Ian B.²,James Oliver F. W.²,Yeaman Stephen J.¹

Affiliation:

1. Department of Biochemistry and Genetics, Newcastle upon Tyne, U.K.

2. Department of Medicine, The Medical School, University of Newcastle upon Tyne, Newcastle upon Tyne, U.K.

Abstract

1. Sera from 76 patients with primary biliary cirrhosis (PBC) and 66 control subjects (53 with chronic liver disease and 13 healthy normal women) were immunoblotted against purified E1 component of bovine pyruvate dehydrogenase complex (PDC) and bacterial PDC. 2. Thirty-one out of seventy-six (41%) sera from PBC patients showed a positive response to bovine E1α, and five of these 31 (7% of total) reacted with bovine E1β. None of the control sera reacted with bovine E1 α or β. 3. None of the PBC sera that recognized bovine E1 subunits reacted with bacterial PDC E1. 4. In the PBC patients there was no correlation between presence of antibodies to E1α and β subunits and histological stage of the disease. 5. Our data demonstrate that the E1α and β components of mammalian PDC are the M2‘d’ and ‘e’ mitochondrial autoantigens, respectively.

Publisher

Portland Press Ltd.

Subject

General Medicine

Link

https://portlandpress.com/clinsci/article-pdf/77/4/365/459951/cs0770365.pdf

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