Stable peptide inhibitors prevent binding of lethal and oedema factors to protective antigen and neutralize anthrax toxin in vivo-Reference-Cited by-同舟云学术

Stable peptide inhibitors prevent binding of lethal and oedema factors to protective antigen and neutralize anthrax toxin in vivo

Published:2006-03-15 Issue:1 Volume:395 Page:157-163
ISSN:0264-6021
Container-title:Biochemical Journal
language:en
Short-container-title:

Author:

Pini Alessandro¹,Runci Ylenia¹,Falciani Chiara¹,Lelli Barbara¹,Brunetti Jlenia¹,Pileri Silvia¹,Fabbrini Monica¹,Lozzi Luisa¹,Ricci Claudia¹,Bernini Andrea¹,Tonello Fiorella²,Dal Molin Federica³,Neri Paolo¹,Niccolai Neri¹,Bracci Luisa¹

Affiliation:

1. Department of Molecular Biology, University of Siena, Via Fiorentina 1, 53100 Siena, Italy

2. Neuroscience Institute, CNR, 35121 Padova, Italy

3. Department of Biomedical Sciences, University of Padova, 35121 Padova, Italy

Abstract

The lethal and oedema toxins produced by Bacillus anthracis, the aetiological agent of anthrax, are made by association of protective antigen with lethal and oedema factors and play a major role in the pathogenesis of anthrax. In the present paper, we describe the production of peptide-based specific inhibitors in branched form which inhibit the interaction of protective antigen with lethal and oedema factors and neutralize anthrax toxins in vitro and in vivo. Anti-protective antigen peptides were selected from a phage library by competitive panning with lethal factor. Selected 12-mer peptides were synthesized in tetra-branched form and were systematically modified to obtain peptides with higher affinity and inhibitory efficiency.

Publisher

Portland Press Ltd.

Subject

Cell Biology,Molecular Biology,Biochemistry

Link

https://portlandpress.com/biochemj/article-pdf/395/1/157/642088/bj3950157.pdf

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