Exacerbated metabolic changes in skeletal muscle of sickle cell mice submitted to an acute ischemia–reperfusion paradigm

Author:

Chatel Benjamin12,Messonnier Laurent A.2,Vilmen Christophe1,Bernard Monique1,Pialoux Vincent3,Bendahan David1

Affiliation:

1. Aix-Marseille Univ, CNRS, CRMBM, Marseille, France

2. Univ Savoie Mont Blanc, Laboratoire Interuniversitaire de Biologie de la Motricité, EA 7424, F-73000 Chambéry, France

3. Univ Lyon, UCBL1, Laboratoire Interuniversitaire de Biologie de la Motricité, EA 7424, Villeurbanne, France

Abstract

Sickle cell disease (SCD) is characterized by painful vaso-occlusive crisis. While there are several metabolic abnormalities potentially associated with muscular ischemia–reperfusion cycles that could be harmful in the context of SCD, the metabolic consequences of such events are still unknown. Ten controls (HbAA), thirteen heterozygous (HbAS), and ten homozygous (HbSS) SCD mice were submitted to a standardized protocol of rest–ischemia–reperfusion of the left leg during which adenosine triphosphate, phosphocreatine, and inorganic phosphate concentrations as well as intramuscular pH were measured using phosphorous magnetic resonance spectroscopy (MRS). Forty-eight hours later, skeletal muscles were harvested. Oxidative stress markers were then measured on the tibialis anterior. At the end of the ischemic period, HbSS mice had a lower pH value as compared with the HbAA and HbAS groups (P<0.01). During the reperfusion period, the initial rate of phosphocreatine resynthesis was lower in HbSS mice as compared with HbAA (P<0.05) and HbAS (P<0.01) animals. No significant difference among groups was observed regarding oxidative stress markers. HbSS mice displayed a higher intramuscular acidosis during the ischemic period while their mitochondrial function was impaired as compared with their HbAA and HbAS counterparts. These metabolic abnormalities could worsen the complications related to the pathology of SCD.

Publisher

Portland Press Ltd.

Subject

General Medicine

Reference62 articles.

1. Sickle-cell disease;Rees;Lancet,2010

2. Sickle-cell haemoglobin polymerization: is it the primary pathogenic event of sickle-cell anaemia?;Vekilov;Br. J. Haematol.,2007

3. Sickle cell disease: selected aspects of pathophysiology;Alexy;Clin. Hemorheol. Microcirc.,2010

4. Sickle cell disease;Piel;N. Engl. J. Med.,2017

5. Acute muscle injury complicating sickle cell crisis;Schumacher;Semin. Arthritis Rheum.,1990

Cited by 1 articles. 订阅此论文施引文献 订阅此论文施引文献,注册后可以免费订阅5篇论文的施引文献,订阅后可以查看论文全部施引文献

同舟云学术

1.学者识别学者识别

2.学术分析学术分析

3.人才评估人才评估

"同舟云学术"是以全球学者为主线,采集、加工和组织学术论文而形成的新型学术文献查询和分析系统,可以对全球学者进行文献检索和人才价值评估。用户可以通过关注某些学科领域的顶尖人物而持续追踪该领域的学科进展和研究前沿。经过近期的数据扩容,当前同舟云学术共收录了国内外主流学术期刊6万余种,收集的期刊论文及会议论文总量共计约1.5亿篇,并以每天添加12000余篇中外论文的速度递增。我们也可以为用户提供个性化、定制化的学者数据。欢迎来电咨询!咨询电话:010-8811{复制后删除}0370

www.globalauthorid.com

TOP

Copyright © 2019-2024 北京同舟云网络信息技术有限公司
京公网安备11010802033243号  京ICP备18003416号-3