Affiliation:
1. La Jolla Cancer Research Foundation, 10901 North Torrey Pines Road, La Jolla, CA 92037, U.S.A.
Abstract
The catalytic activity of human placental alkaline phosphatase (PLAP) and germ cell alkaline phosphatase (GCAP) can be inhibited, through an uncompetitive mechanism, by L-Phe. GCAP is also selectively inhibited by L-Leu. Site-directed mutagenesis of five of the 12 residues which are different in PLAP and GCAP revealed that Gly429 is the primary determinant of GCAP inhibition by L-Leu, and Ser84 and Leu297 play a modulatory role in the inhibition.
Subject
Cell Biology,Molecular Biology,Biochemistry
Cited by
38 articles.
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