Effect of albumin on taurocholate uptake kinetics in rat liver

Author:

Aldini Rita1,Roda Aldo2,Morselli-Labate Antonio Maria1,Simoni Patrizia2,Roda Enrico1,Barbara Luigi1

Affiliation:

1. Clinica Medico III, University of Bologna, S. Orsola Hospital, Bologna

2. Istituto di Scienze Chimiche, Facoltà di Farmacia, University of Bologna, Bologna, Italy

Abstract

1. Isolated rat livers were perfused in a single pass with increasing doses of taurocholate with and without albumin in the perfusion media. 2. The kinetics of taurocholate uptake were thus evaluated. 3. In all the experiments, taurocholate uptake showed Michaelis-Menten kinetics. Increasing the albumin concentration in the medium resulted in an increase in the Km, without effect on the Vmax. When taurocholate and albumin were kept constant (20:1 molar ratio), the Vmax was significantly lower than in the other experiments. 4. These data suggest that taurocholate uptake shows saturation in the absence of albumin and that albumin reduces taurocholate uptake.

Publisher

Portland Press Ltd.

Subject

General Medicine

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