The fatty acid composition of phosphatidylinositol, phosphatidate and 1,2-diacylglycerol in stimulated human neutrophils

Abstract

The fatty acid composition of phosphatidylinositol (PtdIns), phosphatidate (PtdOH) and 1,2-diacylglycerol (DG) was determined in cytochalasin B-treated human neutrophils in the presence and in the absence of formylmethionyl-leucyl-phenylalanine. The compositions of PtdOH and DG in stimulated cells resemble closely that of PtdOH in control cells and are quite different from the composition of PtdIns. DG appears to be produced as a subsequent metabolite of PtdOH and not as an intermediate in the conversion of PtdIns into PtdOH. We conclude that PtdOH is produced directly from a small pool of newly synthesized PtdIns in stimulated neutrophils.