Graphical and statistical analysis of hyperbolic tight-binding inhibition

Abstract

Equations are described for the analysis of enzyme-catalysed reactions in the presence of hyperbolic tight-binding inhibitors using both graphical and non-linear-regression methods. The general character of the equations allows the interpretation of several other kinetic mechanisms. As an example, the tight-binding hyperbolic non-competitive inhibition of human leucocyte elastase by a polysulphated glycosaminoglycan is analysed.