Study on the production of Sophorolipid by Starmerella bombicola yeast using waste fried oil fermentation

Author:

Wang Haifeng1,Gao Ruifang2,Song Xin3,Yuan Xiangdong1,Chen Xiuli4,Zhao Yanling4

Affiliation:

1. Baotou Light Industry Vocational Technical College, Baotou, China

2. Bayannur City modern agriculture and Animal Husbandry development Center, Bayannur City, China

3. Shandong University, shandong, China

4. Baotou Teacher's College, Baotou, China

Abstract

Sophorolipid (SLs) are surface active compounds that have excellent surface-lowering properties. SLs was produced by Starmerella bombicola (CGMCC1576) yeast with sunflower seeds oil, fried waste oil, cooked tung oil and raw tung oil used as hydrophobic carbon sources. The results showed that the strain could use sunflower seed oil and fried waste oil as hydrophobic carbon sources to produce SLs, and the yields were 44.52 and 39.09 gL-1, and it could not use cooked tung oil and raw tung oil. Analysis by high performance liquid chromatography-high resolution mass spectrometry (HPLC-MS/MS) showed that the main composition and structure of SLs produced by fermentation using waste fried waste oil were similar to that of sunflower seed oil as hydrophobic carbon source. The yield of SLs was the highest when the fried waste oil was used as hydrophobic carbon source, glucose 8%, waste oil 6% and yeast 0.3%. When fried waste oil was used as hydrophobic carbon source in a parallel 4-strand fermentation tank (FT), the combination with the largest yield and the most cost saving was that 3% of fried waste oil was added into the initial medium, and another 3% was again added after 72h of fermentation. The total yield of SLs was 121.28 gL-1, and the yield of lactone SLs was 48.07 gL-1.

Funder

scientific research project of higher institution education of Inner Mongolia

Publisher

Portland Press Ltd.

Subject

Cell Biology,Molecular Biology,Biochemistry,Biophysics

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