Hormone-triggered conformational changes within the insulin-receptor ectodomain: requirement for transmembrane anchors

Author:

FLÖRKE Ralf-Rudiger1,SCHNAITH Kerstin1,PASSLACK Waltraud1,WICHERT Marc1,KUEHN Lothar1,FABRY Marlies2,FEDERWISCH Matthias3,REINAUER Hans1

Affiliation:

1. Deutsches Diabetes-Forschungsinstitut an der Heinrich Heine-Universität, Klinische Biochemie2, Auf'm Hennekamp 65, 40225 Düsseldorf, Germany

2. Deutsches Wollforschungsinstitut, Veltmanplatz 8, 52062 Aachen, Germany

3. Institut für Biochemie der Rheinisch-Westfaelischen Technischen Hochschule, Klinikum, Pauwelsstr. 30, 52057 Aachen, Germany

Abstract

Interaction between two αβ half-receptors within the (αβ)2 holoreceptor complex is required for insulin binding with high affinity and for insulin-triggered changes of size and shape. To understand the underlying structure–function relationship, two truncated receptor constructs have been characterized. Reduction in the Stokes radius and increase in the sedimentation coefficient, which are characteristic for wild-type receptors, were entirely lacking for the recombinant human insulin receptor (HIR) ectodomain (HIR-ED). Stokes radii of about 5.8nm and sedimentation coefficients of 10.2S were found for both insulin-bound and free HIR-EDs. However, attaching the membrane anchors to the ectodomain, as with the recombinant membrane-anchored ectodomain (HIR-MAED) construct, was sufficient to restore not only high-affinity hormone binding but also the marked insulin-inducible alterations in hydrodynamic properties. The Stokes radii of HIR-MAED complexes, as assessed by non-denaturing PAGE, decreased upon insulin binding from 9.5nm to 7.9nm. In parallel, the sedimentation coefficient was increased from 9.0S to 9.8S. CD and fluorescence spectroscopy of HIR-MAED revealed only minor insulin-induced changes in the secondary structure. Similarity with wild-type receptors has also been demonstrated by the differential insertion of insulin-bound and free HIR-MAED complexes into artificial bilayer membranes of Triton X-114. The results are consistent with a model of receptor function that ensures a global insulin-triggered reorientation of subdomains within the ectodomain moieties while the secondary structure is essentially retained. For the rearrangement of such subdomains, the transmembrane anchors confer essential structural constraints on the receptor ectodomain.

Publisher

Portland Press Ltd.

Subject

Cell Biology,Molecular Biology,Biochemistry

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