Forsythoside A protects against lipopolysaccharide-induced acute lung injury through up-regulating microRNA-124

Author:

Lu Zibin12ORCID,Yang Huayi12,Cao Huihui12,Huo Chuying12,Chen Yuyao12,Liu Dongyi12,Xie Pei12,Zhou Hongling12,Liu Junshan12ORCID,Yu Linzhong12

Affiliation:

1. Third Level Research Laboratory of State Administration of Traditional Chinese Medicine, School of Traditional Chinese Medicine, Southern Medical University, Guangzhou 510515, P. R. China

2. Guangdong Provincial Key Laboratory of Chinese Medicine Pharmaceutics, Guangzhou 510515. P. R. China

Abstract

Abstract Acute lung injury (ALI) is a life-threatening disease without effective pharmacotherapies, so far. Forsythia suspensa is frequently used in the treatment of lung infection in traditional Chinese medicine. In search for natural anti-inflammatory components, the activity and the underlying mechanism of Forsythoside A (FA) from Forsythia suspensa were explored. In the present paper, BALB/c mice and murine RAW 264.7 cells were stimulated by LPS to establish inflammation models. Data showed that FA inhibited the production of TNF-α and IL-6 and the activation of STAT3 in LPS-stimulated RAW 264.7 cells. Additionally, FA increased the expression level of microRNA-124 (miR-124). Furthermore, the inhibitory effect of FA on STAT3 was counteracted by the treatment of miR-124 inhibitor. Critically, FA ameliorated LPS-induced ALI pathological damage, the increase in lung water content and inflammatory cytokine, cells infiltration and activation of the STAT3 signaling pathway in BALB/c mice. Meanwhile, FA up-regulated the expression of miR-124 in lungs, while administration with miR-124 inhibitor attenuated the protective effects of FA. Our results indicated that FA alleviates LPS-induced inflammation through up-regulating miR-124 in vitro and in vivo. These findings indicate the potential of FA and miR-124 in the treatment of ALI.

Publisher

Portland Press Ltd.

Subject

General Medicine

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