Isoelectric focusing of human liver alkaline phosphatase-Reference-Cited by-同舟云学术

Isoelectric focusing of human liver alkaline phosphatase

Published:1970-06-01 Issue:2 Volume:118 Page:299-302
ISSN:0306-3283
Container-title:Biochemical Journal
language:en
Short-container-title:

Author:

Latner A. L.¹,Parsons Mary E.¹,Skillen A. W.¹

Affiliation:

1. University Department of Clinical Biochemistry, Royal Victoria Infirmary, Newcastle upon Tyne NE1 7RU, U.K.

Abstract

1. Isoelectric focusing of human liver alkaline phosphatase in a sucrose density gradient with LKB Ampholine as carrier ampholytes is described. 2. Problems due to the chelating properties of the ampholytes and the pH gradient were examined. 3. A reactivation procedure to counter these effects was devised that can probably be used for other alkaline phosphatases. 4. The isoelectric point of human liver alkaline phosphatase was found to be pH3.9.

Publisher

Portland Press Ltd.

Link

https://portlandpress.com/biochemj/article-pdf/118/2/299/768432/bj1180299.pdf

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