The active centre of rabbit muscle triose phosphate isomerase. The site that is labelled by glycidol phosphate-Reference-Cited by-同舟云学术

The active centre of rabbit muscle triose phosphate isomerase. The site that is labelled by glycidol phosphate

Published:1971-06-01 Issue:2 Volume:123 Page:163-170
ISSN:0306-3283
Container-title:Biochemical Journal
language:en
Short-container-title:

Author:

Miller Janet C.¹,Waley S. G.¹

Affiliation:

1. Sir William Dunn School of Pathology, University of Oxford, South Parks Road, Oxford OX1 3RE, U.K.

Abstract

1. Glycidol (2,3-epoxypropanol) phosphate is a specific irreversible inhibitor of rabbit muscle triose phosphate isomerase (EC 5.3.1.1); the site of attachment has now been studied. 2. The labelled enzyme was digested with pepsin and a modified peptide isolated. The sequence of the peptide is: Ala-Tyr-Glu-Pro-Val-Trp. 3. It is the glutamic acid residue in this peptide that is labelled: the peptide is thus a γ-glutamyl ester derived from glycerol phosphoric acid. The same site is labelled by a mixture of glycidol and inorganic phosphate. 4. Kinetic and stereochemical features of these reactions are discussed.

Publisher

Portland Press Ltd.

Link

https://portlandpress.com/biochemj/article-pdf/123/2/163/771036/bj1230163.pdf

Cited by 37 articles. 订阅此论文施引文献订阅此论文施引文献，注册后可以免费订阅5篇论文的施引文献，订阅后可以查看论文全部施引文献

1. Biochemical characterisation of triose phosphate isomerase from the liver fluke Fasciola hepatica;Biochimie;2013-11

2. Mechanism for Activation of Triosephosphate Isomerase by Phosphite Dianion: The Role of a Hydrophobic Clamp;Journal of the American Chemical Society;2012-06-06

3. A Paradigm for Enzyme-Catalyzed Proton Transfer at Carbon: Triosephosphate Isomerase;Biochemistry;2012-03-20

4. High resolution crystal structures of triosephosphate isomerase complexed with its suicide inhibitors: The conformational flexibility of the catalytic glutamate in its closed, liganded active site;Protein Science;2011-07-07

5. Enzymatic Catalysis of Proton Transfer at Carbon: Activation of Triosephosphate Isomerase by Phosphite Dianion;Biochemistry;2007-04-20