Affiliation:
1. Department of Biochemistry, University of Hull, Kingston upon Hull HU6 7RX, U.K.
Abstract
The methyl α-glucoside-transport system of Pseudomonas aeruginosa has been characterized with respect to its specificity, energy-dependence, kinetics and regulation. The uptake of glucose involved two components, one of which transported glucose (Km=8μm) and methyl α-glucoside (Km=2.8mm) whereas the other was more complex, involving the extracellular activity of glucose dehydrogenase. Mutants defective in this enzyme have been isolated and characterized. The methyl α-glucoside–glucose-transport system was repressed when the organism was grown in the absence of glucose; the induction of this transport system by glucose, and its activity once induced, were inhibited by products of citrate metabolism.
Cited by
133 articles.
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