The radioimmunoassay of steroid glucuronides. The oestrogen C-3 glucuronides as haptens

Abstract

Antisera were prepared against three related oestrogen ring-A glucuronides, oestrone 3-glucuronide, oestradiol 3-glucuronide and oestriol 3-glucuronide. The corresponding 6,7-3H-labelled conjugates were synthesized as radioligands and the cross-reactions of the antisera against ring-A oestrogen glucuronides and other steroid conjugates were examined. The specificity of the antiserum against oestriol 3-glucuronide was compared with that raised against oestriol 16α-glucuronide, and the measurement of the former conjugate in late-pregnancy urine is discussed.