Interaction of pigeon-liver nicotinamide-adenine dinucleotide kinase with cibacron blue F3GA-Reference-Cited by-同舟云学术

Interaction of pigeon-liver nicotinamide-adenine dinucleotide kinase with cibacron blue F3GA

Published:1976-11-01 Issue:2 Volume:159 Page:441-443
ISSN:0264-6021
Container-title:Biochemical Journal
language:en
Short-container-title:

Author:

Apps D K¹,Gleed C D¹

Affiliation:

1. Department of Biochemistry, University of Edinburgh Medical School, Teviot Place, Edinburgh EH8 9AG, Scotland, U.K.

Abstract

The interaction of pigeon liver NAD kinase with Cibacron Blue F3GA was investigated. By using steady-state rate measurements, spectrophotometric titration and chromatography of the enzyme on immobilized dye, it was shown that binding occurs at two nucleotide sites with different affinities, and also at a site distinct from the substrate-binding region.

Publisher

Portland Press Ltd.

Subject

Cell Biology,Molecular Biology,Biochemistry

Link

https://portlandpress.com/biochemj/article-pdf/159/2/441/568041/bj1590441.pdf

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