The probiotic Lactobacillus coryniformis CECT5711 reduces the vascular pro-oxidant and pro-inflammatory status in obese mice

Author:

Toral Marta1,Gómez-Guzmán Manuel1,Jiménez Rosario12,Romero Miguel12,Sánchez Manuel1,Utrilla María Pilar23,Garrido-Mesa Natividad3,Rodríguez-Cabezas María Elena23,Olivares Mónica4,Gálvez Julio23,Duarte Juan12

Affiliation:

1. Department of Pharmacology, School of Pharmacy, University of Granada, Granada, Spain

2. Instituto de Investigación Biosanitaria de Granada, Granada, Spain

3. CIBER-EHD, Department of Pharmacology, Center for Biomedical Research, University of Granada, Granada, Spain

4. Research Department of Biosearch, Granada, Spain

Abstract

Obesity is associated with intestine dysbiosis and is characterized by a low-grade inflammatory status, which affects vascular function. In the present study, we evaluated the effects of a probiotic with immunomodulatory properties, Lactobacillus coryniformis CECT5711, in obese mice fed on an HFD (high-fat diet). The probiotic treatment was given for 12 weeks, and it did not affect the weight evolution, although it reduced basal glycaemia and insulin resistance. L. coryniformis administration to HFD-induced obese mice induced marked changes in microbiota composition and reduced the metabolic endotoxaemia as it decreased the LPS (lipopolysaccharide) plasma level, which was associated with a significant improvement in gut barrier disruption. Furthermore, it lowered TNFα (tumour necrosis factor α) expression in liver, improving the inflammatory status, and thus the glucose metabolism. Additionally, the probiotic reversed the endothelial dysfunction observed in obese mice when endothelium- and NO (nitric oxide)-dependent vasodilatation induced by acetylcholine in aortic rings was studied. It also restored the increased vessel superoxide levels observed in obese mice, by reducing NADPH oxidase activity and increasing antioxidant enzymes. Moreover, chronic probiotic administration for 2 weeks also improved endothelial dysfunction and vascular oxidative stress induced by in vivo administration of LPS in control mice fed on a standard chow diet. The results of the present study demonstrate an endothelial-protective effect of L. coryniformis CECT5711 in obese mice by increasing NO bioavailability, suggesting the therapeutic potential of this gut microbiota manipulation to prevent vasculopathy in obesity.

Publisher

Portland Press Ltd.

Subject

General Medicine

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