RNAP subunits F/E (RPB4/7) are stably associated with archaeal RNA polymerase: using fluorescence anisotropy to monitor RNAP assembly in vitro-Reference-Cited by-同舟云学术

RNAP subunits F/E (RPB4/7) are stably associated with archaeal RNA polymerase: using fluorescence anisotropy to monitor RNAP assembly in vitro

Published:2009-07-15 Issue:3 Volume:421 Page:339-343
ISSN:0264-6021
Container-title:Biochemical Journal
language:en
Short-container-title:

Author:

Grohmann Dina¹,Hirtreiter Angela¹,Werner Finn¹

Affiliation:

1. Institute for Structural and Molecular Biology, Division of Biosciences, Darwin Building, University College London, Gower Street, London WC1E 6BT, U.K.

Abstract

Archaeal and eukaryotic RNAPs (DNA-dependent RNA polymerases) are complex multi-subunit enzymes. Two of the subunits, F and E, which together form the F/E complex, have been hypothesized to associate with RNAP in a reversible manner during the transcription cycle. We have characterized the molecular interactions between the F/E complex and the RNAP core. F/E binds to RNAP with submicromolar affinity and is not in a dynamic exchange with unbound F/E.

Publisher

Portland Press Ltd.

Subject

Cell Biology,Molecular Biology,Biochemistry

Link

https://portlandpress.com/biochemj/article-pdf/421/3/339/657280/bj4210339.pdf

Reference22 articles.

1. Structural evolution of multisubunit RNA polymerases;Werner;Trends Microbiol.,2008

2. Structure and function of archaeal RNA polymerases;Werner;Mol. Microbiol.,2007

3. Molecular mechanisms of archaeal RNA polymerase;Grohmann;Biochem. Soc. Trans.,2009

4. A recombinant RNA polymerase II-like enzyme capable of promoter-specific transcription;Werner;Mol. Cell,2002

5. Functional divergence in the growing family of RNA polymerases;Landick;Structure,2009

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