Human PHOSPHO1 exhibits high specific phosphoethanolamine and phosphocholine phosphatase activities-Reference-Cited by-同舟云学术

Human PHOSPHO1 exhibits high specific phosphoethanolamine and phosphocholine phosphatase activities

Published:2004-08-10 Issue:1 Volume:382 Page:59-65
ISSN:0264-6021
Container-title:Biochemical Journal
language:en
Short-container-title:

Author:

ROBERTS Scott J.¹,STEWART Alan J.¹,SADLER Peter J.²,FARQUHARSON Colin¹

Affiliation:

1. Roslin Institute, Roslin, Midlothian EH25 9PS, U.K.

2. School of Chemistry, The University of Edinburgh, Edinburgh EH9 3JJ, U.K.

Abstract

Human PHOSPHO1 is a phosphatase enzyme for which expression is upregulated in mineralizing cells. This enzyme has been implicated in the generation of Pi for matrix mineralization, a process central to skeletal development. PHOSPHO1 is a member of the haloacid dehalogenase (HAD) superfamily of Mg2+-dependent hydrolases. However, substrates for PHOSPHO1 are, as yet, unidentified and little is known about its activity. We show here that PHOSPHO1 exhibits high specific activities toward phosphoethanolamine (PEA) and phosphocholine (PCho). Optimal enzymic activity was observed at approx. pH 6.7. The enzyme shows a high specific Mg2+-dependence, with apparent Km values of 3.0 μM for PEA and 11.4 μM for PCho. These results provide a novel mechanism for the generation of Pi in mineralizing cells from PEA and PCho.

Publisher

Portland Press Ltd.

Subject

Cell Biology,Molecular Biology,Biochemistry

Link

https://portlandpress.com/biochemj/article-pdf/382/1/59/718100/bj3820059.pdf

Reference48 articles.

1. Fourier transform infrared characterization of mineral phases formed during induction of mineralization by collagenase-released matrix vesicles in vitro;Sauer;J. Biol. Chem.,1988

2. Morphological and biochemical characterization of mineralizing primary cultures of avian growth plate chondrocytes: evidence for cellular processing of Ca2+ and Pi prior to matrix mineralization;Wu;J. Cell. Biochem.,1995

3. Matrix vesicles and calcification;Anderson;Curr. Rheumatol. Rep.,2003

4. Association of matrix acid and alkaline phosphatases with mineralization of cartilage and endochondral bone;Roach;Histochem. J.,1999

5. Alkaline and acid phosphatases in bone cells serve as phosphohydrolases at physiological pH in vivo: a histochemical implication;Nakano;Connect. Tissue Res.,2003

Cited by 109 articles. 订阅此论文施引文献订阅此论文施引文献，注册后可以免费订阅5篇论文的施引文献，订阅后可以查看论文全部施引文献

1. Regional difference in the distribution of alkaline phosphatase, PHOSPHO1, and calcein labeling in the femoral metaphyseal trabeculae in parathyroid hormone-administered mice;Journal of Oral Biosciences;2024-09

2. Integrated proteomics and metabolomics analysis of sclerosis-related proteins and femoral head necrosis following internal fixation of femoral neck fractures;Scientific Reports;2024-06-08

3. Differential effects of the lipidic and ionic microenvironment on NPP1's phosphohydrolase and phosphodiesterase activities;Biochimica et Biophysica Acta (BBA) - Biomembranes;2024-04

4. The biochemistry of mineralizing extracellular vesicles. Part I: The role of phosphatases;Mineralizing Vesicles;2024

5. Immunolocalization of Enzymes/Membrane Transporters Related to Bone Mineralization in the Metaphyses of the Long Bones of Parathyroid-Hormone-Administered Mice;Medicina;2023-06-20