The use of sodium perchlorate in deproteinization during the preparation of nucleic acids-Reference-Cited by-同舟云学术

The use of sodium perchlorate in deproteinization during the preparation of nucleic acids

Published:1973-11-01 Issue:3 Volume:135 Page:559-561
ISSN:0264-6021
Container-title:Biochemical Journal
language:en
Short-container-title:

Author:

Wilcockson John¹

Affiliation:

1. Department of Applied Genetics, John Innes Institute, Colney Lane, Norwich NOR 70F, U.K.

Abstract

Sodium perchlorate in high concentrations will remove from solution the detergent sodium dodecyl sulphate and protein complexed with it. This and the failure of proteins to be precipitated by ethanol from solutions containing a high concentration of sodium perchlorate can be utilized as efficient, rapid and simple deproteinization procedures during the preparation of nucleic acids.

Publisher

Portland Press Ltd.

Subject

Cell Biology,Molecular Biology,Biochemistry

Link

https://portlandpress.com/biochemj/article-pdf/135/3/559/561551/bj1350559.pdf

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