The cytotoxicity of 3-bromopyruvate in breast cancer cells depends on extracellular pH

Author:

Azevedo-Silva João1,Queirós Odília12,Ribeiro Ana1,Baltazar Fátima34,Young Ko H.5,Pedersen Peter L.6,Preto Ana1,Casal Margarida1

Affiliation:

1. Centre of Molecular and Environmental Biology (CBMA), Department of Biology, University of Minho, Campus de Gualtar, Braga 4710-057, Portugal

2. CESPU, Instituto de Investigação e Formação Avançada em Ciências e Tecnologias da Saúde, Rua Central de Gandra, 1317, 4585-116, Gandra, PRD, Portugal

3. Life and Health Sciences Research Institute (ICVS), School of Health Sciences, University of Minho, Campus de Gualtar, Braga 4710-057, Portugal

4. ICVS/3B's - PT Government Associate Laboratory, Braga, 4710-057, Portugal

5. KoDiscovery, LLC, University of Maryland BioPark, Suite 502 E & F, 801 West Baltimore St., Baltimore, MD 21201, U.S.A.

6. Departments of Biological Chemistry and Oncology, Member at Large, Sidney Kimmel Comprehensive Cancer Center, John Hopkins University, School of Medicine, Baltimore, MD 21205-2185, U.S.A.

Abstract

Transport of the anti-cancer agent 3-bromopyruvate (3BP) in breast cancer cells is mediated by monocarboxylate transporter (MCT)-1 activated by glycosylated chaperone cluster of differentiation (CD) 147. The extracellular acidic pH increases the affinity for 3BP uptake enhancing its selective cytotoxic effect in tumour cells.

Publisher

Portland Press Ltd.

Subject

Cell Biology,Molecular Biology,Biochemistry

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