Validation of a simple radiochemical assay measuring hydrolysis of choline-labelled microsomal phosphatidylcholine by phospholipase C. pH-dependence-Reference-Cited by-同舟云学术

Validation of a simple radiochemical assay measuring hydrolysis of choline-labelled microsomal phosphatidylcholine by phospholipase C. pH-dependence

Published:1976-12-15 Issue:3 Volume:160 Page:475-479
ISSN:0264-6021
Container-title:Biochemical Journal
language:en
Short-container-title:

Author:

Cater B R¹,Trivedi P¹,Hallinan T¹

Affiliation:

1. Department of Biochemistry and Chemistry, Royal Free Hospital School of Medicine, 8 Hunter Street, London WC1N 1BP, U.K.

Abstract

Selective hydrolysis of phosphatidylcholine species, which are selectively radioactively labelled in vivo, does not appear to interfere with a radiochemical assay for hydrolysis of microsomal phosphatidylcholine by C-type phospholipases from Bacillus cereus or Clostridium perfringens. Both phospholipases substantially hydrolysed phosphatidylcholine over the pH range 4.0-10.0.

Publisher

Portland Press Ltd.

Subject

Cell Biology,Molecular Biology,Biochemistry

Link

https://portlandpress.com/biochemj/article-pdf/160/3/475/570675/bj1600475.pdf

Cited by 4 articles. 订阅此论文施引文献订阅此论文施引文献，注册后可以免费订阅5篇论文的施引文献，订阅后可以查看论文全部施引文献

1. Effects of Activation Agents on Uridine Diphosphate Glucuronosyltransferase Activity towards Acceptor Substrates of Different Octanol/Buffer Partition Coefficients;Biochemical Society Transactions;1978-12-01

2. Phospholipid content and activity of pure uridine diphosphate-glucuronyltransferase from rat liver;Biochemical Journal;1978-06-01

3. Phospholipid dependence of UDP-glucuronyltransferase;Biochimica et Biophysica Acta (BBA) - Biomembranes;1978-02

4. Re-activation of Guinea-Pig Liver Uridine Diphosphate Glucuronosyltransferase with 1-Monoacyl Phosphatidylcholine after Extensive Phospholipid Deletion at 5°C with Pure Bacillus cereus Phospholipase C;Biochemical Society Transactions;1977-02-01