Adenosine transport in peripheral blood lymphocytes from Lesch-Nyhan patients

Author:

TORRES Rosa J.1,DeANTONIO Isabel1,PRIOR Carmen1,PUIG Juan G.2

Affiliation:

1. Clinical Biochemistry Division, ‘La Paz’ University Hospital, Paseo de la Castellana 261, 28046 Madrid, Spain

2. Internal Medicine Division, ‘La Paz’ University Hospital, Paseo de la Castellana 261, 28046 Madrid, Spain

Abstract

We postulated that adenosine function could be related to some of the neurological features of Lesch–Nyhan syndrome and therefore characterized adenosine transport in PBLs (peripheral blood lymphocytes) obtained from Lesch–Nyhan patients (PBLLN) and from controls (PBLC). Adenosine transport was significantly lower in PBLLN when compared with that in PBLC and a significantly lower number of high affinity sites for [3H]nitrobenzylthioinosine binding were quantified per cell (Bmax) in PBLLN when compared with that in PBLC. After incubation with 25 µM hypoxanthine, adenosine transport was significantly decreased in PBLLN with respect to PBLC. Hypoxanthine incubation lowers [3H]nitrobenzylthioinosine binding in PBLC, with respect to basal conditions, but does not affect it in PBLLN. This indicates that hypoxanthine affects adenosine transport in control and hypoxanthine–guanine phosphoribosyltransferase-deficient cells by different mechanisms.

Publisher

Portland Press Ltd.

Subject

Cell Biology,Molecular Biology,Biochemistry

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