Towards understanding the phosphorylation code of tau-Reference-Cited by-同舟云学术

Towards understanding the phosphorylation code of tau

Published:2012-07-20 Issue:4 Volume:40 Page:698-703
ISSN:0300-5127
Container-title:Biochemical Society Transactions
language:en
Short-container-title:

Author:

Lippens Guy¹,Amniai Laziza¹,Wieruszeski Jean-Michel¹,Sillen Alain¹,Leroy Arnaud²,Landrieu Isabelle¹

Affiliation:

1. CNRS UMR 8576 University of Lille 1, 59655 Villeneuve d'Ascq, France

2. Laboratoire de Biochimie Appliquée, Faculté de Pharmacieà Châtenay-Malabry, University of Paris XI, 5 Rue Jean-Baptiste Clément, 92296 Châtenay-Malabry Cedex, France

Abstract

We describe our efforts to combine in vitro enzymatic reactions with recombinant kinases to phosphorylate the neuronal tau protein, and NMR spectroscopy to unravel the resulting phosphorylation pattern in both qualitative and quantitative manners. This approach, followed by functional assays with the same samples, gives access to the complex phosphorylation code of tau. As a result, we propose a novel hypothesis for the link between tau (hyper)phosphorylation and aggregation.

Publisher

Portland Press Ltd.

Subject

Biochemistry

Link

https://portlandpress.com/biochemsoctrans/article-pdf/40/4/698/485893/bst0400698.pdf

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