Affiliation:
1. The Dyson Perrins Laboratory, University of Oxford, Oxford OX1 3QY, U.K.
Abstract
The pH-dependences of the kinetic parameters kcat. and Km for the triose phosphate isomerase reaction were determined in each direction. Apparent pKa values of 6.0 and 9.0 are observed in the dependences of kcat./Km. The pH-dependences of kcat. are sigmoid, with apparent pKa values of about 6.0. The results are interpreted in terms of a single base on the enzyme providing an efficient proton-shuttling mechanism for the isomerization.
Cited by
163 articles.
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