Compartmental oxidation of thiol–disulphide redox couples during epidermal growth factor signalling-Reference-Cited by-同舟云学术

Compartmental oxidation of thiol–disulphide redox couples during epidermal growth factor signalling

Published:2005-02-22 Issue:2 Volume:386 Page:215-219
ISSN:0264-6021
Container-title:Biochemical Journal
language:en
Short-container-title:

Author:

HALVEY Patrick J.¹²,WATSON Walter H.³,HANSEN Jason M.¹,GO Young-Mi¹,SAMALI Afshin²,JONES Dean P.¹

Affiliation:

1. Department of Medicine, Division of Pulmonary, Allergy and Critical Care Medicine, School of Medicine, Room 225, Emory University, 615 Michael Street, Atlanta, GA 30322, U.S.A.

2. Department of Biochemistry and National Centre for Biomedical Engineering Science, National University of Ireland, Galway, University Road, Galway, Ireland

3. Department of Environmental Health Sciences, Bloomberg School of Public Health, Room E7545, Johns Hopkins University, 615 N. Wolfe Street, Baltimore, MD 21205, U.S.A.

Abstract

Exogenously added ROS (reactive oxygen species) cause generalized oxidation of cellular components, whereas endogenously generated ROS induced by physiological stimuli activate discrete signal transduction pathways. Compartmentation is an important aspect of such pathways, but little is known about its role in redox signalling. We measured the redox states of cytosolic and nuclear Trx1 (thioredoxin-1) and mitochondrial Trx2 (thioredoxin-2) using redox Western blot methodologies during endogenous ROS production induced by EGF (epidermal growth factor) signalling. The glutathione redox state was measured by HPLC. Results showed that only cytosolic Trx1 undergoes significant oxidation. Thus EGF signalling involves subcellular compartmental oxidation of Trx1 in the absence of a generalized cellular oxidation.

Publisher

Portland Press Ltd.

Subject

Cell Biology,Molecular Biology,Biochemistry

Link

https://portlandpress.com/biochemj/article-pdf/386/2/215/716727/bj3860215.pdf

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