Assay of adenosine 3′-phosphate 5′-sulphatophosphate in hepatic tissues

Abstract

A fluorimetric assay, based on the ability of boiled hepatic extracts to support the sulphO-conjugation of harmol, was used to demonstrate and quantify PAdoPS (adenosine 3′-phosphate 5′-sulphatophosphate) present in liver. A stoicheiometric relationship was established between the sulphate conjugate formed and the ‘active sulphate’ utilized. Guinea-pig, rat, mouse and rabbit livers contain 3.3, 2.9, 0.8 and 0.5 mumol of PAdoPS/100 G wet wt. respectively.