Affiliation:
1. Physiologisch-Chemisches Institut der Universitat Tiubingen, Hoppe-Seyler-Strasse 4, 7400 Tiubingen, Federal Republic of Germany.
Abstract
Ins(1,3,4,5)P4 has been suggested to be involved in cellular Ca2+ signalling. A receptor from pig cerebellar membranes, which binds InsP4 with high affinity and selectivity [Donié & Reiser (1989) FEBS Lett. 254, 155-158], has been solubilized and purified about 20,000-fold by chromatography using CM-cellulose, heparin-agarose and hydroxyapatite. The InsP4 receptor, identified by SDS/PAGE, had an apparent molecular mass of 42 kDa and bound 4.6 nmol of InsP4 per mg of protein, with a dissociation constant of 5.6 nM.
Subject
Cell Biology,Molecular Biology,Biochemistry
Cited by
63 articles.
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