Use of DT40 conditional-knockout cell lines to study chromosomal passenger protein function-Reference-Cited by-同舟云学术

Use of DT40 conditional-knockout cell lines to study chromosomal passenger protein function

Published:2010-11-24 Issue:6 Volume:38 Page:1655-1659
ISSN:0300-5127
Container-title:Biochemical Society Transactions
language:en
Short-container-title:

Author:

Fant Xavier¹,Samejima Kumiko¹,Carvalho Ana¹²,Ogawa Hiromi¹,Xu Zhenjie¹³,Yue Zuojun¹⁴,Earnshaw William C.¹,Ruchaud Sandrine⁵

Affiliation:

1. Wellcome Trust Centre for Cell Biology, University of Edinburgh, Edinburgh EH9 3JR, Scotland, U.K.

2. Ludwig Institute for Cancer Research, Department of Cellular and Molecular Medicine, 9500 Gilman Drive, La Jolla, CA 92093-0653, U.S.A.

3. Department of Molecular and Cellular Physiology, Stanford University School of Medicine, 279 Campus Drive, Stanford, CA 94305-5345, U.S.A.

4. Cell and Developmental Biology, The University of Dundee, Dundee DD1 5EH, Scotland, U.K.

5. CNRS USR3151, Station Biologique de Roscoff, place G. Teissier, 29680 Roscoff, France

Abstract

The CPC [chromosomal passenger complex; INCENP (inner centromere protein), Aurora B kinase, survivin and borealin] is implicated in many mitotic processes. In the present paper we describe how we generated DT40 conditional-knockout cell lines for incenp1 and survivin1 to better understand the role of these CPC subunits in the control of Aurora B kinase activity. These lines enabled us to reassess current knowledge of survivin function and to show that INCENP acts as a rheostat for Aurora B activity.

Publisher

Portland Press Ltd.

Subject

Biochemistry

Link

https://portlandpress.com/biochemsoctrans/article-pdf/38/6/1655/549926/bst0381655.pdf

Reference50 articles.

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3. INCENP binds the Aurora-related kinase AIRK2 and is required to target it to chromosomes, the central spindle and cleavage furrow;Adams;Curr. Biol.,2000

4. Exploring the functional interactions between Aurora B, INCENP, and survivin in mitosis;Honda;Mol. Biol. Cell,2003

5. The chromosomal passenger complex is required for chromatin-induced microtubule stabilization and spindle assembly;Sampath;Cell,2004

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