A member of the eukaryotic subtilisin family (PC3) has the enzymic properties of the type 1 proinsulin-converting endopeptidase-Reference-Cited by-同舟云学术

A member of the eukaryotic subtilisin family (PC3) has the enzymic properties of the type 1 proinsulin-converting endopeptidase

Published:1992-07-15 Issue:2 Volume:285 Page:391-394
ISSN:0264-6021
Container-title:Biochemical Journal
language:en
Short-container-title:

Author:

Bailyes E M¹,Shennan K I J²,Seal A J²,Smeekens S P³,Steiner D F³,Hutton J C¹,Docherty K²

Affiliation:

1. Department of Clinical Biochemistry, University of Cambridge, Addenbrookes Hospital, Hills Road, Cambridge CB2 2QR, U.K.

2. Department of Medicine, University of Birmingham, Queen Elizabeth Hospital, Birmingham. B15 2TH, U.K.

3. Howard Hughes Medical Institute and Department of Biochemistry and Molecular Biology, University of Chicago, Chicago, IL 60637, U.S.A.

Abstract

PC3, a mammalian homologue of the yeast subtilisin-like proteinase Kex2, was expressed in Xenopus oocytes and its activity was characterized. PC3 cleaved human proinsulin at one of the two dibasic sites (KTRR32 but not LQKR65). The specificity, inhibitor profile, pH optimum (5.5) and Ca(2+)-dependence (K0.5 = 2.5-3 mM) paralleled those of the insulin-granule type 1 endopeptidase activity, suggesting a role for PC3 in the conversion of prohormones.

Publisher

Portland Press Ltd.

Subject

Cell Biology,Molecular Biology,Biochemistry

Link

https://portlandpress.com/biochemj/article-pdf/285/2/391/607749/bj2850391.pdf

Cited by 131 articles. 订阅此论文施引文献订阅此论文施引文献，注册后可以免费订阅5篇论文的施引文献，订阅后可以查看论文全部施引文献

1. Loci for insulin processing and secretion provide insight into type 2 diabetes risk;The American Journal of Human Genetics;2023-02

2. Distinct Dibasic Cleavage Specificities of Neuropeptide-Producing Cathepsin L and Cathepsin V Cysteine Proteases Compared to PC1/3 and PC2 Serine Proteases;ACS Chemical Neuroscience;2022-01-05

3. Molecular evolution of PCSK family: Analysis of natural selection rate and gene loss;PLOS ONE;2021-10-28

4. Synergistic Effects of Genetic Variants of Glucose Homeostasis and Lifelong Exposures to Cigarette Smoking, Female Hormones, and Dietary Fat Intake on Primary Colorectal Cancer Development in African and Hispanic/Latino American Women;Frontiers in Oncology;2021-10-07

5. Genetic Signatures of Glucose Homeostasis: Synergistic Interplay With Long-Term Exposure to Cigarette Smoking in Development of Primary Colorectal Cancer Among African American Women;Clinical and Translational Gastroenterology;2021-10