Phosphorylation of high-molecular-weight membrane protein species in Chinese-hamster ovary cells in culture: effect of 6-N,2′-O-dibutyryladenosine 3′:5′-cyclic monophosphate plus testosterone (Short Communication)

Abstract

Growth of Chinese-hamster ovary cells in [32P]phosphate and [3H]leucine and subsequent assay of the plasma membranes reveals phosphorylation in two protein regions corresponding to molecular weights of 280000 and 195000. Culture in the presence of the 6-N,2-O′-dibutyryl derivative of cyclic AMP plus testosterone does not stimulate [32P]-phosphate incorporation, but determines a modification in the qualitative pattern of phosphorylation.