A new, highly sensitive method for the detection and quantification of penicillin-binding proteins-Reference-Cited by-同舟云学术

A new, highly sensitive method for the detection and quantification of penicillin-binding proteins

Published:1993-04-01 Issue:1 Volume:291 Page:19-21
ISSN:0264-6021
Container-title:Biochemical Journal
language:en
Short-container-title:

Author:

Galleni M¹,Lakaye B¹,Lepage S¹,Jamin M¹,Thamm I¹,Joris B¹,Frère J M¹

Affiliation:

1. Laboratoire d'Enzymologie and Centre d'lngéniérie des Protéines, Université de Liége, Institut de Chimie, B6, B-4000 Sart-Tilman, Belgium

Abstract

A new method for the identification and quantification of penicillin-binding proteins is described which uses fluorescein-coupled penicillins. It allows the rapid detection of 0.2 pmol with the naked eye and 2 fmol with the help of an A.L.F. automatic DNA sequencer. Direct labelling can also be performed on whole bacterial cells.

Publisher

Portland Press Ltd.

Subject

Cell Biology,Molecular Biology,Biochemistry

Link

https://portlandpress.com/biochemj/article-pdf/291/1/19/610741/bj2910019.pdf

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