A p.m.r. isotope-exchange method for studying the kinetic properties of dehydrogenases in intact cells-Reference-Cited by-同舟云学术

A p.m.r. isotope-exchange method for studying the kinetic properties of dehydrogenases in intact cells

Published:1982-03-15 Issue:3 Volume:202 Page:573-579
ISSN:0264-6021
Container-title:Biochemical Journal
language:en
Short-container-title:

Author:

Simpson R J,Brindle K M,Brown F F,Campbell I D,Foxall D L

Abstract

A method to determine the activity of dehydrogenases in an intact-cell system is described. The method involves the use of n.m.r. to monitor bulk isotope exchange. The approach is illustrated by application to the isotope equilibration of pyruvate and lactate as catalyzed by lactate dehydrogenase in intact erythrocytes. Particular problems peculiar to bulk isotope exchange and its observation by n.m.r. are considered.

Publisher

Portland Press Ltd.

Subject

Cell Biology,Molecular Biology,Biochemistry

Link

https://portlandpress.com/biochemj/article-pdf/202/3/573/577039/bj2020573.pdf

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