Affiliation:
1. Departments of Chemistry and Applied Biology, University of Wales Institute of Science and Technology, Cardiff CF1 3NU, U.K.
Abstract
Methods are described for the first reported successful isolation of the soluble form of the membrane-bound myo-inositol dehydrogenase(s) from Acetomonas oxydans. Conditions for optimum yields of active enzyme in a crude membrane-free protein extract were established. The relevant conditions are (a) cell rupture by solid-shearing, (b) solubilization of the complete 60000g pellet with sodium deoxycholate at pH7.2, (c) rapid separation of the released protein from sodium deoxycholate by gel chromatography.
Subject
Cell Biology,Molecular Biology,Biochemistry
Cited by
8 articles.
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