Single-molecule tracking (SMT): a window into live-cell transcription biochemistry-Reference-Cited by-同舟云学术

Single-molecule tracking (SMT): a window into live-cell transcription biochemistry

Published:2023-03-06 Issue:2 Volume:51 Page:557-569
ISSN:0300-5127
Container-title:Biochemical Society Transactions
language:en
Short-container-title:

Author:

Dahal Liza¹²³,Walther Nike¹³,Tjian Robert¹²³,Darzacq Xavier¹³^ORCID,Graham Thomas G.W.¹²³

Affiliation:

1. 1Department of Molecular and Cell Biology, University of California, Berkeley, Berkeley, U.S.A.

2. 2Howard Hughes Medical Institute, University of California, Berkeley, Berkeley, U.S.A.

3. 3Li Ka Shing Center for Biomedical & Health Sciences, University of California, Berkeley, Berkeley, U.S.A.

Abstract

How molecules interact governs how they move. Single-molecule tracking (SMT) thus provides a unique window into the dynamic interactions of biomolecules within live cells. Using transcription regulation as a case study, we describe how SMT works, what it can tell us about molecular biology, and how it has changed our perspective on the inner workings of the nucleus. We also describe what SMT cannot yet tell us and how new technical advances seek to overcome its limitations. This ongoing progress will be imperative to address outstanding questions about how dynamic molecular machines function in live cells.

Publisher

Portland Press Ltd.

Subject

Biochemistry

Link

https://portlandpress.com/biochemsoctrans/article-pdf/51/2/557/945770/bst-2022-1242c.pdf

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