The specific assay of arylsulphatase C, a rat liver microsomal marker enzyme-Reference-Cited by-同舟云学术

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The specific assay of arylsulphatase C, a rat liver microsomal marker enzyme

Published:1972-06-01 Issue:2 Volume:128 Page:331-336
ISSN:0306-3283
Container-title:Biochemical Journal
language:en
Short-container-title:

Author:

Milsom D. W.¹,Rose F. A.¹,Dodgson K. S.¹

Affiliation:

1. Department of Biochemistry, University College, P.O. Box 78, Cardiff CF1 1XL, U.K.

Abstract

Conditions based on previous assays with potassium p-acetylphenyl sulphate have been established for the specific assay of arylsulphatase C in rat tissues. The enzyme has optimum activity with 40mm substrate at pH8.0 in the presence of 0.1m-phosphate buffer. Under these conditions arylsulphatase C can be assayed without interference from the other arylsulphatase enzymes present and is useful as a marker for the endoplasmic reticulum in cell-fractionation studies.

Publisher

Portland Press Ltd.

Link

https://portlandpress.com/biochemj/article-pdf/128/2/331/777157/bj1280331.pdf

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