Characterization of Saccharomyces cerevisiae deficient in expression of phospholipase D-Reference-Cited by-同舟云学术

Characterization of Saccharomyces cerevisiae deficient in expression of phospholipase D

Published:1996-02-15 Issue:1 Volume:314 Page:15-19
ISSN:0264-6021
Container-title:Biochemical Journal
language:en
Short-container-title:

Author:

ELLA Krishna M.¹,DOLAN Joseph W.²,QI Chen¹,MEIER Kathryn E.¹

Affiliation:

1. Departments of Cell and Molecular Pharmacology, Medical University of South Carolina, 171 Ashley Avenue, Charleston, SC 29425-2251, U.S.A.

2. Departments of Microbiology/Immunology, Medical University of South Carolina, 171 Ashley Avenue, Charleston, SC 29425-2251, U.S.A.

Abstract

A gene encoding phospholipase D (PLD) in Saccharomyces cerevisiae was identified. The 195 kDa product of PLD1 has 24% overall sequence identity with a plant PLD. Expression of yeast PLD activity was eliminated by one-step gene disruption. Yeast haploids lacking PLD activity were deficient in growth on non-fermentable carbon sources. Diploids lacking expression of PLD1 were unable to sporulate.

Publisher

Portland Press Ltd.

Subject

Cell Biology,Molecular Biology,Biochemistry

Link

https://portlandpress.com/biochemj/article-pdf/314/1/15/618586/bj3140015.pdf

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